Dilution Experiment



This is the second part of my second day in the STEM program: "Dilution experiment." Also, I learned how to properly mix a solute in a solvent. (Sept. 13th, 2019)

CALCULATE DILUTION:

The first dilution experiment we did was to practice using micropipettes. Later on, we used this equation for another experiment. That is why I included it here:

Equation: M1V1=M2V2


During the experiment, we pipetted different amounts with different pipettes. First, we pipetted from the solution with a high concentration of food colorant to a tube with water. Then, we pipetted from the second solution to another tube with water, and so on. We ended up with 5 tubes with different colors (that showed different dilutions): red, dark orange, orange, yellow, pale yellow.

Later on, we learned how to properly mixed solutions. We mixed glucose in water for future media.

  • We measured .25g of glucose. We threw to the trash the excess because you never put back to the container (cross-contamination)
  • We first get a flask that holds more than what we want. (1 1/2 Rule)
  • First, we poured 100 mL of DI water and mixed that with the glucose.
  • When is mixed, we pour the rest of the DI water (150 ml). 
This method helps to throughly mix any solution into a solvent 

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