Preparing LB media.



The following days in the lab we learned about P-Glow transformation. On this day, we prepared media, made labels and learned how to prepare before any experiment. (Sept. 17th, 2019)

The most important things we always have to do every time we go into the lab are:
  • sanitize the table where we are going to work (with ethanol 70%)
  • wash our hands before we start working.  
  • Have a list ready for the materials we will need during the day. 
This will prevent cross-contamination and mistakes during the experiment (we will have everything close to us while working in the experiment).

LABELS:


In order to keep track of all the experiments, everything has to be labeled correctly. 
THE LABELS HAVE TO BE AROUND THE DISH, BUT NOT IN THE MIDDLE (IT SHOULDN'T COVER WHAT IT CONTAINS)

Labels have:
  • Name of the person (or Initials)
  • Date of when it was done.
  • What it contains 

MEDIA:

There are different recipes for media, the one work with is this one:

LB Agar (500 mL) USE 2 LITTER FLASK (1 1/2 RULE)

  • 5 grams of NaCl
  • 5 grams of Tryptone
  • 2.5 grams of Yeast Extract.
  • 7.5 grams of agar
To prepare LB broth is the same recipe, but without agar. 


Once everything is mixed, we placed a stir bar inside of the flask. Then we placed the flask on a hot plate. When is ready, we took out the stir bar.



























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