SPRG. SEM. (1/17/20) Gels

In the first week of the Spring semester, we worked with one gel with the DNA samples of Chad, and  we helped to fill some flasks

GEL:

First, we did a 1.7% gel for 3 DNA samples. We work with samples 19, 20 and 24.

Here, we were preparing the 1.7% gel after mixing TAE Buffer and Agarose. We used to use a microwave to heat it up in time sections. Now, we were using a hot plate. Every few seconds, we hold the flask (using a glove) and move it, so the heat wouldn't be concentrated in just the bottom. 






After we had the Gel solid, we prepared the dye for the DNA samples. 
We used 2μL of dye, 5μL of distilled water, 5μL of DNA sample. For the Blank, we used 5μL more of distilled water instead of DNA. 


 

This is after we finish pipetting the DNA samples into the gel. This is the time when we just need to check the DNA doesn't go past the arrow. 

              
    


For some reason, I was excited to see the bubbles that day.....It took me a while to focus the camera in these bubbles

Results:

                      
 




FLASKS:

Here we filled these 5 flasks with 5 mL of distilled water. This was a normal task in the lab; however, I learn about the tape every glass container has to have before been autoclaved. 


The tape is green before been autoclaved, then when is out is black.

                                   








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