SPRG. SEM. (2/1/20) DNA Isolation and PCR

On Saturday of the third week, we finished doing DNA isolation for the rest of the DNA samples and prepared them for PCR.


The samples we worked on were Sample 14 (Pimensis) and Sample 10 (Indicus).


When we did the Nanodrop for these bacteria DNA, the results were not good for Indicus (they were negative). Here are the results:



As a result, we only prepared the PCR tubes for samples 1,7,8 and 14. We pipetted 5 microliters of these DNA samples into the PCR tubes that were already prepared. Also, we pipetted 5 microliters of autoclaved distilled water for the blank :



After the PCR tubes were ready, they were placed into the DNA amplifier for 1 hour and a half.



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