SPRG. SEM. (1/31/20) DNA Isolation

In the third week, we did DNA isolation, so we could do a PCR on the next day.

The bacteria that was used for this DNA isolation was the one that was inoculated on January 24th. 
There were eight samples of bacteria (1, 7, 8, 10, 11, 14, 17, 20). On Tuesday, January 24th, Gram Stains were with this bacteria in order to see if they were not contaminated. Samples 11, 17 and 20 were contaminated.
We performed DNA isolation on samples 1, 7 and 8 on this day.

  • Sample 1 was Aquaticus 
  • Sample 7 was Gobiensis
  • Sample 8 was Grandis


Unfortunately, I did not take pictures while we did them, but here is a picture of the results: 




Comments

Post a Comment

Popular posts from this blog

Protein: Q13231 (CHIT1)

Image
This semester, I'm focusing on CHIT1.  CHIT1 is a gene and encodes a protein called Chitotriosidase-1, and is a member of the chitinase family. This is selectively expressed in activated tissue macrophages. As a biomarker  (it indicates the  severity or presence of some disease state) is the best  to monitor Gaucher disease from the three most commonly used. Also, it is a marker for macrophage activation in acute ischemic stroke. Chromosomal location  Cytogenetic Location: 1q32.1. 1q    = The long (q) arm of the chromosome 1 32.1 =  It is the position in the arm. Molecular Location: base pairs 203,216,079 to 203,229,673  Data and Picture Reference: Genetics Home Reference.  August 17th, 2020. CHIT1 gene, Chitinase1. Retrieved from: https://ghr.nlm.nih.gov/gene/CHIT1#resources Function:  Chitinases wo rks in both innate and adaptive immunity.  They are necessary for the following functions: Degrades chitin, chitotriose and...
Read more

CULTURAL INTELLIGENCE

Something that I learn about cultural intelligence and cultural responsiveness is to first have cultural openness and cultural awareness. From what I understood one has to be open to learn about other people's culture with an open mind. However, this doesn't mean to be invasive or assume somebody's cultural background.  I like the definition of  Business professors P. Christopher Earley and Elaine Mosakowski about cultural intelligence. They define it as “an outsider’s seemingly natural ability to interpret someone’s unfamiliar and ambiguous gestures the way that person’s compatriots would.”  This means to have the ability to relate to people from other cultures by empathizing and actively working to understand customs and norms of other cultures besides our own  ( What Is Cultural Intelligence and Why Is It Key to Business? – Nationwide , n.d.). Now, according to the National Center for Culturally Responsive Educational Systems (NCCREST), “cultural respons...
Read more

First Day! Streak Method, Gram Stain Test and Microscope.

Image
First day in the STEM-TRAIN program. I learned about the “Streak Plate Method”, the “Gram Stain Test” and how to use a microscope. (Sept. 10th, 2019) V-Fisheri bacteria At the beginning of the class, Shawn, a senior student, explained to me the experiment he was working on. He was working with V-fisheri bacteria that have as bioluminescent properties. The goal of his experiment is to find some components that can stop the bacteria from releasing AHL molecules. The V-fischeri bacteria is a gram-negative bacterium found in marine environments When it multiplies and it is ready to communicate with each other, it releases QS molecules. When these molecules are released, the biofilm glows. If the biofilm doesn’t glow there is “something” that stops the communication among these bacteria Pathogenic bacteria also release molecules when it tries to communicate with the others; however, these molecules are toxins. The V-fisheri experiment is successful, it can be applied to p...
Read more