SPRG. SEM. (2/8/20) DNA Isolation

On Saturday, February 8th, we worked did a second DNA with Sample 14.



  These are the results of the DNA isolation done on 2/7/20. The results were not good, so we decided to do another DNA isolation of Sample 14.


 We decided to use another kit different from last time. This one was the DNeasy UltraClean Microbial Kit. The steps in this kit were different from the Wizard Plus SV Miniprep. We continue using the same culture from Friday, thus now it was a 48hrs.


We have used this kit multiple times. We usually repeat the first step (centrifuge 1.8 ml of microbial culture in order to have a pellet), However, this time we only worked with 1.8 ml instead of 5.4.

We worked with only one bacteria culture, so we had to balance it with the same tubes but with distilled water instead. 


These were the results of this DNA Isolation:








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